SUMMER SCHOOL OF SINGLE CELL

FROM THE LAB TO DATA ANALYSIS

September 4 – 11, 2024, Pilsen, Czech Republic

Charles University, University of West Bohemia,

University of Warsaw, London South Bank University, University of Pennsylvania, University of Milan, University of Geneva, Masaryk University and Heidelberg University

Organizers and supervisors

Monika Holubová

Monika Holubová

Group leader of the Laboratory of Tumor Biology and Immunotherapy, Biomedical Center, Faculty of Medicine in Pilsen, Charles University

Dr. Holubová main interest is study of immune and cancer cells interactions. She is responsible for the translation research and development of the new immunotherapeutic approaches for the hematological disorders and immune dysregulation. She is an expert in cell production, flow cytometry and cell sorting including single-cell isolation.

Lucie Houdová

Lucie Houdová

Group leader of the Clinical Data Science of European Centre of Excellence NTIS, Faculty of Applied Sciences, University of West Bohemia

Dr. Houdová main interest is research of IT tools for applications of personalized and precise medicine, primarily focused on the area of hematology and oncology. She is experienced in data analysis, methods and information systems design, computer modeling for decision support in medicine and technical practice. Currently, she is vice-chairman of the ELIXIR CZ Board (European Life-Science Infrastructure for Biological Information – Czech node).

School patron

Milena Králíčková

Milena Králíčková

Rector of Charles University

Prof. Králíčková is a rector of Charles University. She was a key scientific leader during the development of a Biomedical center of Faculty of Medicine, was deeply involved in the establishment of 4EU+ alliance and strongly supports the activities within Elixir CZ. Her huge encouragement was the basis for the beginning of the collaboration between organizers and supervisors leading to the organization of this unique summer school.

Main wet lab supervisor

Valentina Caputo

Valentina Caputo

Senior Lecturer of Biomedical Science, School of Applied Sciences, London South Bank University

Dr. Caputo is focused on the study of epigenetic mechanisms in human disease and the development of epigenetic therapeutic approaches. Her particular interest is in investigating the molecular mechanisms by which nuclear architecture, transcriptional regulation and epigenetics can be causative of a disease phenotype and how this can be targeted for therapy of human disorders.

Main data analysis supervisor

Aleksander Jankowski

Aleksander Jankowski

Assistant Professor, Faculty of Mathematics, Informatics and Mechanics, University of Warsaw

Dr. Jankowski is focused on bioinformatics, regulatory genomics and machine learning. He uses and develops computational methods to interpret the three-dimensional organization of the genome, dissect its regulatory impact on gene expression, and integrate next-generation sequencing data from single-cell and population-level experiments. He is also involved in teaching programming and data science skills to non-computational researchers.

External supervisors

Antonia Rotolo

Antonia Rotolo

Assistant Professor, University of Pennsylvania Veterinary Cancer Center, Philadelphia, PA

Prof. Rotolo is a physician-scientist whose work concentrates on invariant Natural Killer T Cells (iNKTs) and their potential for adoptive cell therapy. Her work combines single-cell transcriptomics with both in vitro and in vivo models to dissect iNKT biology and mechanisms of function and develop novel iNKT-based immunotherapies. The goal of Dr. Rotolo’s research is to provide the foundation for clinical development of allogeneic iNKT therapies for cancer and transplant patients.

Bartosz Wojtaś

Bartosz Wojtaś

Assistant Professor, Head of Laboratory of Sequencing, Nencki Institute of Experimental Biology, Polish Academy of Sciences

Dr. Wojtaś is focused on transcriptomics, epigenomics and computational biology. He uses and builds analytical pipelines for next-generation sequencing data, including single-cell transcriptomic data. His main scientific interests are related with molecular oncology, especially with epigenomic bases of gene expression regulation in glioblastoma.

Honorary supervisors

Iros Barozzi

Iros Barozzi

Junior Group Leader at Medical University of Vienna & Honorary Research Fellow at Imperial College London

Dr. Barozzi is a computational biologist interested in regulatory genomics. He combines integrative analytical approaches with machine learning to investigate the role of the non-coding genome in embryonic development and in the onset and progression of hormone-dependent tumours. He is currently using single-cell transcriptomics and epigenetic profiling to study the role of transcriptional heterogeneity in drug-resistant luminal breast cancers. He si also ELIXIR-UK member.

Local supervisors

Pavel Ostašov

Pavel Ostašov

Senior researcher in Biomedical Center, Faculty of Medicine in Pilsen, Charles University

Dr. Ostašov main interest is analysis of single cells in cancer and their heterogeneity and liquid biopsies. He is experienced in library preparation, next generation sequencing and data analysis. He is also member of ELIXIR CZ.

Diana Macečková

Diana Macečková

PhD student, Biomedical Center, Faculty of Medicine, Charles University

Diana focuses on study of cancer cell chemoresistance and is very experienced in cell culture technique, cell selection and processing.

Robin Klieber

Robin Klieber

PhD student, Biomedical Center, Faculty of Medicine, Charles University

Robin focuses on characterization of cell free molecules and NGS analysis including sample and library processing for single-cell techniques.

Kateřina Wolf

Kateřina Wolf

PhD student, Faculty of Applied Sciences, University of West Bohemia

Kateřina focuses on data analysis and framework design, especially related to the processing of sequencing and clinical data.

E-learning supervisor

Miroslava Čedíková

Miroslava Čedíková

Assistant Professor in Biomedical Center and Department of Physiology, Faculty of Medicine in Pilsen, Charles University and Faculty of Health Care Studies, University of West Bohemia

Dr. Čedíková works mostly with pre-graduate students and is a specialist in preparation of the teaching materials. In research, she is interested in immune cells and their role in the pathophysiology of endometriosis. She works with advanced microscopy including life cell imaging.

Other supervisors

Eliška Jandová

Jana Skálová

Jiří Fatka