PROGRAM
Day 1 (Wed, Sept. 4) – Welcome and Lectures
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08:00 – 08:45
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Registration
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08:45 – 09:00
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Welcome and opening
Milena Králíčková (Rector of Charles University)
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09:00 – 10:30
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PART 1
Chair: Valentina Caputo (London South Bank University) and Lenka Bešše (Masaryk University)
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Licensing innate immune response in cancer therapies
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Domenico Mavilio (University of Milan)
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30 min
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New era of single cell transcriptomics
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Agnieszka Ciesielska (10X Genomics)
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30 min
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Microenvironmental control of lymphocyte responses
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Ana Stojanovic (University of Heidelberg)
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30 min
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10:30 – 10:50
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Coffee break
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10:50 – 12:00
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PART 2
Chair: Valentina Caputo (London South Bank University) and Antonia Rotolo (University of Pennsylvania)
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Targeting tumor heterogeneity with engineered immune cells
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Denis Migliorini (University of Geneva)
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30 min
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Mastering Cell Fate: The Crucial Role of NF-YA Alternative Splicing in mESC Differentiation
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Diletta Dolfini (University of Milan)
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30 min
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Presentation of Consortium for iNKT Research and Therapy (CiRT)
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Monika Holubová (Charles University)
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10 min
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12:00 – 13:00
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Lunch break
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13:00 – 14:30
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PART 3
Chair: Pavel Ostašov (Charles University) and Bartosz Wojtaś (Polish Academy of Sciences)
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Bone marrow microenvironment-driven resistance to proteasome inhibition in multiple myeloma
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Lenka Bešše (Masaryk University and Kantonsspital St. Gallen)
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30 min
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Advancing iNKT therapies through comparative and computational biology
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Antonia Rotolo (University of Pennsylvania)
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30 min
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BD Rhapsody™ Single-Cell Analysis System Fast track single-cell research without compromise
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Vadir Lopez-Salmeron (BD Bioscience)
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30 min
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14:30 – 15:00
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Coffee break
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15:00 – 16:15
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PART 4
Chair: Pavel Ostašov (Charles University) and Lucie Houdová (University of West Bohemia)
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REST transcription factor in glioblastoma: KAISO transcription factor is entering the stage
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Bartosz Wojtaś (Nencki Institute of Experimental Biology, Polish Academy of Sciences)
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30 min
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Approach for Advancing Transcriptomics: Single Cell and Spatial Solutions
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Michal Kolář (Institute of Molecular Genetics, Czech Academy of Sciences)
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30 min
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Needs and Possibilities of Data Science for Medicine
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Roman Mouček (University of West Bohemia)
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15 min
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17:30 – 20:00
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Networking with welcome drink
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Day 2 (Thu, Sept. 5) – Basic training for wet lab and data analysis
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Morning session
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Group 1
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Basic training for wet lab (supervisors: Valentina Caputo, Robin Klieber)
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Group 2
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Basic training for data analysis – Introduction to R (supervisors: Lucie Houdová, Kateřina Wolf)
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Group 1 + 2
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Summary of Introduction to R
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Afternoon session
Supervisors: Lucie Houdová, Kateřina Wolf
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R programming basic training
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Introduction to data analysis
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Day 3 (Fri, Sept. 6) – Wet lab day 1
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Group A
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Single-cell sample preparation and 10XGenomics training (supervisors: Valentina Caputo, Pavel Ostašov, Diana Macečková, Robin Klieber)
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Group B
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Cell sorting and single-cell analysis using Rhapsody system (supervisors: Antonia Rotolo, Monika Holubová, Jiří Šinkora)
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Day 4 (Sat, Sept. 7) – Wet lab day 2
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Group A
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Cell sorting and single-cell analysis using Rhapsody system (supervisors: Antonia Rotolo, Monika Holubová, Jiří Šinkora)
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Group B
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Single-cell sample preparation and 10XGenomics training (supervisors: Valentina Caputo, Pavel Ostašov, Diana Macečková, Robin Klieber)
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Day 5 (Sun, Sept. 8) – Day-off
Organized tour through Pilsen with visit to the brewery and beer tasting.
Day 6 (Mon, Sept. 9) – Data analysis
Supervisors: Aleksander Jankowski, Bartosz Wojtaś; co-supervisors: Kateřina Wolf, Pavel Ostašov
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Morning session
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Introduction to scRNA-seq data analysis
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Quality control
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Normalization and dimensionality reduction
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Afternoon session
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Cell clustering
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Marker gene identification
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Day 7 (Tue, Sept. 10) – Data analysis
Supervisors: Aleksander Jankowski, Bartosz Wojtaś; co-supervisors: Kateřina Wolf, Pavel Ostašov
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Morning session
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Cell type annotation
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Cell cycle annotation
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Afternoon session
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Multimodal data analysis
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Introduction to group projects
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Day 8 (Wed, Sept. 11) – Data analysis and barbecue party
Supervisors: Aleksander Jankowski, Bartosz Wojtaś; co-supervisors: Kateřina Wolf, Pavel Ostašov
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Morning session
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Afternoon session
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Presentation of group projects
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Conclusion and certification
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Barbecue party
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Note: Unless specified directly in the program Lunch and coffee breaks will be determined according to the current situation.
